Figure 1.

Evaluation of hematopoiesis-derived cell populations in the bone marrow (BM) and peripheral organs of Pparg^Δ/Δ mice. (A) Representative photographs of the spleens and livers of Pparg^Δ/Δ (γ^Δ/Δ) mice (lower panels) and littermate control (CTL) mice (upper panels). (B) Spleen and liver weight in grams (top panels) and expressed as % of the body weight (bottom panels). (C) Hematoxylin & eosin staining of spleen sections; White pulp areas are circled by a dotted line. The white arrow indicates one of the numerous megakaryocytes present in the red pulp in Pparg^Δ/Δ mice. The black bar indicates 200 micrometers. (D) Total hematopoietic cell numbers in the BM (2 tibias and 2 femurs from each mouse), spleen, and liver of control and Pparg^Δ/Δ mice. Mean ± SEM, n = 7–8 mice per genotype. (E) Total numbers of mature hematopoietic cell subsets in the BM (left panel), spleen (middle panel) and liver (right panel) of control (dark bars) and Pparg^Δ/Δ (light bars) mice. (F) Same as in (E), with cell numbers expressed as a % of the total cell number in the corresponding organ. T cells (CD3⁺); B cells (B220⁺); Gran: granulocytes (Gr1⁺CD11b⁺), Macs: macrophages (Gr1⁻CD11b⁺), Ebs: erythroblasts (Ter119⁺CD71⁺); Mean ± SEM n = 7–8 mice per genotype. All significant p-values are indicated above the corresponding bars.