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. 2018 Nov 13;9:2626. doi: 10.3389/fimmu.2018.02626

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Copyright © 2018 Voogdt, Merchant, Wagenaar and van Putten.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of codon optimized alligator and crocodile TLR15. Immunoblot analyses of HEK293 cells and VH-2 cells expressing C-terminal FLAG-tagged chicken (gaga), anolis (anca), alligator (almi), codon optimized alligator (almi opt.), crocodile (cpro), or codon optimized crocodile (crpo opt.) TLR15. Mature TLR15 is ~140 kDa. Beta-actin was detected to confirm equal loading of HEK293 and VH-2 total protein onto SDS-PAGE gel. Results are representative of at least two independent experiments.