Table 2.

K_d values of protein–protein interactions within the posttranslational cpSRP-pathway of Arabidopsis thaliana

Interaction	Method	Kd [µM]	References
cpSRP43/Alb3
43/Alb3 C-terminus	FA	12–18	Liang et al. (2016)
43/Alb3 C-terminus	ITC	5.1	Horn et al. (2015)
43/Alb3 C-terminus	ITC	9.7–13.2	Falk et al. (2010); Falk and Sinning (2010a, 2010b)
43/Alb3 C-terminus	ITC	0.094	Lewis et al. (2010)
cpSRP43/LHCP
43/LHCP	LS	0.17/1.5	Liang et al. (2016)
43/LHCP + 54M		0.26
43/LHCP	FA	0.14–0.3	Jaru-Ampornpan et al. (2010)
43/L18	ITC	0.322	Gao et al. (2015)
43/L18 + RRKRp10		0.107
43/L18	ITC	1.17	Stengel et al. (2008)
43/L11	FA	0.022	Liang et al. (2016)
43/L11 + Alb3 C-terminus		0.011
cpSRP43/cpSRP54
43/54	MST	0.05	Ziehe et al. (2016)
43/54	FCS	0.095	Gao et al. (2015)
43/54M	SPR	0.0025	Hermkes et al. (2006)
cpSRP54/cpFtsY
54/FtsY - PG	FRET	2.34	Chandrasekar and Shan (2017)
54/FtsY + PG		0.13
54/FtsY*	FRET	0.77	Chandrasekar et al. (2017)
54NG/FtsY*		12

Enlisted are the dissociation constants (K_d values) of protein complexes of the posttranslational cpSRP-pathway. Additionally, the methods by which the K_d values were determined as well as possible variations of the experimental settings are shown

FA fluorescence anisotropy, FCS fluorescence correlation spectroscopy, FRET forster resonance energy transfer, ITC isothermal titration calorimetry, LA light scattering, MST microscale thermophoresis, SPR surface plasmon resonance spectroscopy

* measured in the presence of 0.01 % Nikkol (mimicking of the effect of lipids)