Table 1.
A summary of the four carbofuran immunoassays.
| Parameter | Format | |||
|---|---|---|---|---|
| Format I | Format II | Format III | Format IV | |
| BFNH-OVA (ng/mL) | 0.5 | 0.25 | — | — |
| MAbs (ng/mL) | 0.057 | — | 0.35 | 1.42 |
| The secondary antibody | Peroxidase-labeled goat anti-mouse immunoglobulins diluted 1:20000 | — | — | 1 μg/mL Goat anti-mouse immunoglobulins |
| Antibody-HRP | — | 1:80 | — | — |
| BFNH-HRP | — | — | 1:800 | 1:400 |
| Step | 5 | 3 | 3 | 4 |
| Assay time(h) | 4 | 1 | 1.5 | 2 |
| IC50 (ng/mL) | 11.63 | 18.49 | 6343.38 | >10000 |
| Limit detection (10% inhibition) (ng/mL) | 0.007 | 0.11 | 0.066 | 3.58 |
| Linearity detection range (ng/mL) | IC20-IC80 | IC20-IC80 | IC20-IC50 | IC8-IC15 |
| 0.08-1694.93 | 0.65-692.64 | 9.82-6343.38 | 0.74-188.92 | |
— Not applicable