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. 2008 Dec 3;13(12):2975–2985. doi: 10.3390/molecules13122975

Figure 3.

Figure 3

TNF-α-induced MMP-9 activity and expression was inhibited by MEK and p38 inhibitors and TNF-α-induced AP-1 DNA binding activity were suppressed by berberine in MDA-MB-231 cells. (A) After serum-starvation for 16 h, cells were pretreated with 10 μM UO126, SB203580 and SP600126, respectively, for 30 min and then treated with 20 ng/mL TNF-α for 24 h. MMP-9 activity and expression were analyzed by Zymography (upper band) and Western blotting (lower band), respectively. (B) After serum-starvation for 16 h, cells were pretreated with 10 μM berberine for 1 h and then treated with 20 ng/mL TNF-α for the indicated times. (B) Using the nucleus protein, AP-1 DNA binding activity was analyzed by EMSA as described in Materials and Methods. These results were representative of three independent experiments. Values shown are means ± SEM. * P < 0.01 vs. control. † P < 0.01 vs. TNF-α-treated cells.Con; control, U; UO126, SB; SB203580, SP; SP600126.