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. 2018 Oct 18;23(4):237–248. doi: 10.1007/s10911-018-9416-0

Fig. 2.

Fig. 2

ER and PR expression and R5020 response in engineered human DCIS.COM cells. Lentivirus transduction and cell sorting was used to stably express different combinations of ERα/PR including PR (A or B isoforms), ERα alone or both ERα and PR in DCIS.COM cells. STR DNA fingerprinting was done by the CCSG-funded Characterized Cell Line Core at M.D. Anderson Cancer Center (NCI # CA016672) to validate the cell lines as breast cancer epithelial cell origin. Expression of PR or ER is shown by immunoblot analysis in panel (a). Immunofluorescent labeling of ER+/PR+ DCIS.COM cells demonstrates that ER and PR are each expressed in nuclei of the majority of cells, Scale bar: 50 μm (b). These engineered cell lines are responsive to the synthetic progestin R5020 or 17β estradiol (E2) in terms of induction of known target gene expression by qRT-PCR after 24-h hormone treatment (c)