Fig. 1.

Reactivity of Anti-HLA-I mAbs (W6/32, TFL-006, HC-10) against all HLA-A (n = 28), HLA-B (n = 44) and HLA-C (n = 13) antigens coated on LS (Cat # LS1A04, Lot 10) (Blue) and LC (Cat # 265100R, Lot# 3005613) (Red) HLA-I beadsets. The MFI cutoff used for a positive reaction is 1000. Note that W6/32 bound to 100% of antigens on both beadsets. HC-10 bound to fewer HLA-A antigens (10/28 for LC and 13/28 for LS), most HLA-B antigens (95%) and all HLA-C antigens (100%). In contrast, TFL-006 bound to most of the alleles on LS but failed to bind to any antigens on LC beadsets, confirming the paucity of β2 m-free HLA heavy chains in LC