Table 1.
Reactivity of ESRD patients’ sera to the unique HLA antigens coated on LC and LS beadsets. Each beadset have several unique HLA-I and HLA-II antigens not found in other vendor’s beadsets. Antigens on the beadsets were detected with two different secondary antibodies, namely FcMonoIgG & IgHPolyFab. Sera IDs are given if an antigen is reactive; Antigens recognized by one of the secondary antibodies is indicated as Mono + or Poly +
| HLA-Class I Beadsets | |||
| Unique to LIFECODE (n = 12) | MGH sera | Unique to LABScreen (n = 12) | MGH sera |
| A*02:02 | 008/015 (015 only Poly +) | A*02:06 | 008/15 |
| A*02:05 | [008] | A*30:02 | (007 only Poly+] |
| B*07:03 | None | A*34:01 | [007] |
| B*15:18 | None | B*13:01 | None |
| B*27:03 | None | B*15:10 | None |
| B*35:08 | None | B*40:06 | None |
| B*82:01 | [007] | B*51:02 | 001/7/27, all Poly+ |
| Cw*04:03 | 002/007/008 (MGH-002 only Mono +) | B*57:03 | 001/2/8/11/27 (001 only Poly+) |
| Cw*07:01 | None | B*82:02 | None |
| Cw*08:02 | None | Cw*03:02 | [007] |
| Cw*12:02 | [007] | Cw*12:03 | 007/024, (024 only Poly+) |
| Cw*18:01 | None | Cw*18:02 | 007/25 |
| HLA-Class II Beadsets | |||
| Unique to LIFECODE (n = 34) | MGH sera | Unique to LABScreen (n = 34) | MGH sera |
| DRB1*03:03 | (007 o + S15:S41nly Poly +)] | DRB1*09:02 | 001/018/019/027] (011/019/027 only Mono*) |
| DRB1*08:02 | 007/011 | DRB1*14:02 | [007] |
| DRB1*11:03 | 007/011 | DRB1*14:54 | [007] |
| DRB1*13:05 | 007/011 | DRB4*01:03 | 005/018/023 |
| DRB1*14:03 | 007/011 (007 only Poly+) | DQB1*02:01\DQA1*03:01 | 00100/6/008/018/027 (001/006 only Mono+ |
| DRB1*14:04 | [007] | DQB1*02:01\DQA1*04:01 | 007/018 |
| DQB1*02:02\DQA1*03:02 | 008/018 | DQB1*03:01\DQA1*02:01 | 007/008/011/018 |
| DQB1*02:02\DQA1*05:01 | 007/018 | DQB1*03:03\DQA1*03:01 | None |
| DQB1*03:01\DQA1*03:02 | 007/008/011 | DQB1*03:01\DQA1*05:03 | 007/008/011 |
| DQB1*03:01\DQA1*05:01 | 007/008/011 | DQB1*03:01\DQA1*05:05 | 007/008/011 |
| DQB1*03:03\DQA1*04:01 | 007/008/011 | DQB1*03:03\DQA1*02:01 | 007/008/011/018 |
| DQB1*03:03\DQA1*06:01 | 007/008/011 | DQB1*04:01\DQA1*03:03 | 007/008 |
| DQB1*04:01\DQA1*04:01 | [007] | DQB1*04:02\DQA1*02:01 | None |
| DQB1*04:01\DQA1*05:01 | [007] | DQB1*06:02\DQA1*01:01 | 008/124 (024 only Poly+) |
| DQB1*04:02\DQA1*03:01 | 007/009 | DQB1*06:09\DQA1*01:02 | [008] |
| DQB1*04:02\DQA1*06:01 | [007] | DPB1*03:01\DPA1*01:05 | None |
| DQB1*05:01\DQA1*01:02 | [008] | DPB1*03:01\DPA1*02:01 | [024 only Poly+] |
| DQB1*05:03\DQA1*01:04 | [008] | DPB1*04:01\DPA1*01:03 | None |
| DQB1*06:01\DQA1*01:04 | [008] | DPB1*06:01\DPA1*02:01 | None |
| DQB1*06:01\DQA1*02:01 | 008/018 | DPB1*10:01\DPA1*02:02 | None |
| DPB1*01:01\DPA1*02:02 | None | DPB1*11:01\DPA1*01:03 | [024 only Poly+] |
| DPB1*01:01\DPA1*03:01 | None | DPB1*11:01\DPA1*02:02 | [010/015/024 (024 only Poly+)] |
| DPB1*04:01\DPA1*01:03 | None | DPB1*13:01\DPA1*02:02 | [001/005/006/016 all Mono+, [024] |
| DPB1*04:01\DPA1*02:01 | None | DPB1*13:01\DPA1*03:01 | [024 only Poly+] |
| DPB1*04:01\DPA1*02:02 | None | DPB1*18:01\DPA1*01:04 | None |
| DPB1*04:01\DPA1*03:01 | None | DPB1*18:01\DPA1*01:05 | None |
| DPB1*04:01\DPA1*04:01 | None | DPB1*18:01\DPA1*02:01 | None |
| DPB1*04:02\DPA1*03:01 | None | DPB1*19:01\DPA1*01:03 | [002/006/016/024/027] (002/006/027 Mono+) |
| DPB1*05:01\DPA1*03:01 | None | DPB1*09:01\DPA1*02:01 | None |
| DPB1*11:01\DPA1*02:01 | None | DPB1*20:01\DPA1*03:01 | None |
| DPB1*13:01\DPA1*04:01 | None | DPB1*23:01\DPA1*02:01 | 001/002/019/020/023/025, (002/025 Mono+) |
| DPB1*18:01\DPA1*01:03 | None | DPB1*28:01\DPA1*01:03 | None |
| DPB1*19:01\DPA1*02:01 | None | DPB1*28:01\DPA1*01:05 | None |
| DPB1*28:01\DPA1*02:02 | None | DPB1*28:01\DPA1*04:01 | None |