Fig. 2.
Heat shock conditioning induces DAMP production in GBCCL. The levels of ATP (a) or HMGB1 (b) were evaluated in the supernatants from heat shock-treated or control cells. c Representative histograms showing the extracellular expression levels of translocated calreticulin (eCRT) in heat shock-treated (dark grey) or control (light grey) melanoma and GBC cells. White histograms indicate isotype control staining. The percentage of eCRT positive (eCRTpos) for each condition is shown. d Statistical analysis of eCRT translocation induced by heat shock in GBCCL. Bars represent averages and standard deviations of three (b–d) or five-seven (a) measurements of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001