Fig. 3.

Heat shock-conditioned GBCCL lysate mixtures, but not lysates from individual cell lines, induce differentiation of activated monocytes into mature DCs. Surface expression of HLA-DR, CD80, CD86 (a, b), and HLA-ABC, CD83, and CCR7 (c) were evaluated by flow cytometry on activated monocytes (AM) incubated or not for 24 h with 100 µg/mL of heat shock-conditioned tumor lysates generated from individual GBCCL (a) or mixtures (M1-M8) of three different GBCCL (b, c). Bars represent the average and SD of the fold induction of the integrated MFI (iMFI: % positive cells × geoMFI of positive cells) for each marker relative to AM from at least three independent experiments. Evaluated cell lysates mix were made as follows: M1 (24TKB + GBd1 + G415); M2 (2TKB + 24TKB + GBd1); M3 (1TKB + 2TKB + 24TKB); M4 (OCUG1 + GBd1 + G415); M5 (2TKB + G415 + OCUG1); M6 (NOZ + OCUG 1 + G415); M7 (1TKB + 14TKB + 24TKB); and M8 (24TKB + OCUG1 + G415). *p < 0.05; **p < 0.01; ***p < 0.001