Fig. 1.

Flow cytometric analysis of T regulatory cells (Treg). First, lymphocytes were gated (R1) on the basis of FSC (forward scatter channel) vs. SSC (side scatter channel) signals. CD4⁺ lymphocytes were then gated and the proportion of CD25⁺FOXP3⁺ cells among the CD4⁺ cells was estimated. a Lymphocyte gating. b Isotype control. c Sample analysis of FOXP3⁺ in CD4⁺ cells (Treg—3.64%). d 99.8% of CD4⁺FOXP3⁺ cells co-express CD25 antigen