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. 2018 Jun 27;66(6):443–452. doi: 10.1007/s00005-018-0515-9

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Representative flow cytometric analysis of Th17 cells. First, lymphocytes were gated (R1) on the basis of FSC (forward scatter channel) vs. SSC (side scatter channel) signals. The second step was gating (R2) of CD4⁺ lymphocytes and subsequently estimation of IL-17A⁺ cells among CD4⁺ cells. a Lymphocyte gating. b Gating of CD3⁺CD4⁺ lymphocytes. c Isotype control. d Sample analysis of IL-17A⁺ in CD3⁺CD4⁺ cells (R3—2.5%)