Fig. 3.

Lack of PTX3 impairs vessel proliferation 14 and 28 days after MCAo. a i KI-67 (red) and lectin (blue) co-immunohistochemistry of ipsilateral or contralateral hemispheres of penumbra region in wild type (WT) or pentraxin 3 knockout (PTX3 KO) mice. Scale bar 50 μM. a ii, b i, c i, d i Number of Ki-67-positive (+) blood vessels in the ipsilateral or contralateral hemisphere of penumbra region of WT or PTX3 KO mice was evaluated using Image J software. a iii, b ii, c ii, d ii quantification of number of KI-67⁺ vessels as a percentage (%) of total number of vessels present using ImageJ software. a–d Statistical analyses determined by repeated measures two-way ANOVA followed by Sidak corrected post-hoc analysis (ns P > 0.05, ** P ≤ 0.01, *** P ≤ 0.001). All data expressed as mean ± SD (n = 5–10)