Fig. 4.

PTX3 KO mice have impaired angiogenesis 28 days after MCAo. a i, b i PECAM-1 and VEGFR2 co-immunohistochemistry, PECAM-1 (green), and VEGFR2 (red), 28 days after MCAo of ipsilateral (stroke) or contralateral hemispheres of penumbra and core regions as labelled. Scale bar 50 μM. Percentage (%) area of a ii, b ii PECAM-1, a iii, b iii VEGFR2, and a iv, b iv VEGFR2 as % of PECAM-1% area staining of ipsilateral and contralateral hemispheres of penumbra and core regions were quantified with ImageJ software. a–b Statistical analyses determined by using repeated measures two-way ANOVA followed by Sidak corrected post hoc analysis (ns P > 0.05, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001). All data expressed as mean ± SD (n = 9–10)