Fig. 5.

Vessel-based extracellular matrix (ECM) proteins collagen IV (col IV), laminin, and ECM receptor integrin-β1expression correlate with previously observed reduced vasculature in pentraxin 3 knockout (PTX3 KO) mice 28 days after MCAo. a i, c i, e i Vasculature labelled with lectin (blue) and co-immunostaining with col IV (red), laminin (red), or integrin-β1 (green), respectively, in ipsilateral and contralateral hemispheres of penumbra region as labelled. Scale bar 50 μM. b i col IV (red) and d i laminin (red) immunostaining of ipsilateral and contralateral hemispheres of the core region as labelled. Scale bar 50 μM. Percentage (%) area staining of col IV (a ii, b ii) laminin (c ii, d ii) or integrin-β1 (e ii) in ipsilateral and contralateral hemisphere was quantified using ImageJ software. % area staining of a iii col IV, c iii laminin, or e iii integrin-β1 as a % of lectin % area staining in the ipsilateral or contralateral hemisphere of the penumbra region was assessed with ImageJ software. Scale bar 50 μM. a–e Statistical analyses were assessed using repeated measures two-way ANOVA followed by Sidak corrected post hoc analysis (ns P > 0.05, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001). All data are presented as mean ± SD (n = 8–10)