Fig. 7.

Pentraxin 3 (PTX3) is neuroprotective 28 days after MCAo. i Immunohistochemistry of NeuN (blue) positive (+) neurons in ipsilateral or contralateral hemispheres of penumbra region in wild type (WT) or PTX3 knockout (KO) mice as labelled. Scale bar 50 μM. ii Number of NeuN⁺ neurons in the ipsilateral and contralateral hemispheres of the penumbra region of WT and PTX3 KO mice were counted using ImageJ software. The number of NeuN⁺ neurons present in the ipsilateral hemisphere was calculated as a percentage of NeuN⁺ neurons counted in the corresponding contralateral hemisphere for WT and PTX3 KO mice. Statistical analyses assessed using repeated measures two-way ANOVA followed by Sidak corrected post hoc analysis (* P ≤ 0.05, *** P ≤ 0.001). All data expressed as mean ± SD (n = 8–10)