Figure 2.

TLC analysis of CHE sub-fractions.

(a) Six sub-fractions of the CHE fraction separated by silica gel column chromatography. Plates (b, c) were developed with chloroform-petroleum ether-methanol-formic acid = 2:6:0.5:0.1 (v/v), but (b) was photographed under a UV lamp, while (c) was stained with 5% vanillin-concentrated sulfuric acid reagents and heated at 105°C for 5 min. Lane 1 represents CHE, lanes 2-7 represented fractions CHE1~CHE6.