Table 2.
Summary of the rs numbers, designed primers, amplicon length before and after enzyme digestion, and the specific restriction enzymes according to their allelic genotypes for all the seven XRCC3 SNPs
| rs number | Primer sequencea | Restriction enzyme | Amplicon length (bp) | Allelic genotypes and enzymatic fragment sizes |
|---|---|---|---|---|
|
| ||||
| rs1799794 | F: 5′-CACACTGCGGTCTTGCAGTG-3′ | BtsCI | 505 | G: 505 bp |
| R: 5′-CAGGCTGGGTCTGGATACAA-3′ | A: 289+216 bp | |||
| rs45603942 | F: 5′-GGGATGCAGGTTCAACTGAC-3′ | AluI | 352 | C: 352 bp |
| R: 5′-AACTTGGACTGTGTCAAGCA-3′ | T: 187+165 bp | |||
| rs861530 | F: 5′-CCGAGGAACGTGCTGAACTT-3′ | FatI | 497 | G: 497 bp |
| R: 5′-CTCCCTAACAGCCTCCATGT-3′ | A: 293+204 bp | |||
| rs3212057 | F: 5′-CCATGACCGCAGGCACTTGT-3′ | HpyCH4III | 455 | G: 455 bp |
| R: 5′-AGAACGCGACAAGGATGGTA-3′ | A: 235+220 bp | |||
| rs1799796 | F:5′-GGAACCAGTTGTGTGAGCCT-3′ | AluI | 430 | G: 430 bp |
| R: 5′-CCTGGTTGATGCACAGCACA-3′ | A: 226+204 bp | |||
| rs861539 | F: 5′-GACACCTTGTTGGAGTGTGT-3′ | FatI | 358 | C: 358 bp |
| R: 5′-GTCTTCTCGATGGTTAGGCA-3′ | T: 200+158 bp | |||
| rs28903081 | F: 5′-CTGCTTCCTGTTTCTCAGGT-3′ | BstUI | 198 | A: 198 bp |
| R: 5′-GCACTGATCGTGTAGGAACA-3′ | G: 102+96 bp | |||
Notes:
a
F and R indicate forward and reverse primers, respectively.
Abbreviation: SNPs, single-nucleotide polymorphisms.