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. 2018 Nov 20;14(11):e1007767. doi: 10.1371/journal.pgen.1007767

Fig 10. Anatomical and functional interactions between LK and insulin signaling.

Fig 10

(A) Expression of trans-Tango components [31] using Lk-GAL4 (from K. Asahina and D. Anderson) generates a presynaptic signal (labeled with anti-GFP antibody) in the subesophageal ganglion (SEG) and the lateral horn, and a postsynaptic signal (labeled with anti-HA antibody) in the SEG and pars intercerebralis. (B) Higher magnification of the SEG showing the presynaptic signals and the lack of post-synaptic signal in median neurosecretory cell bodies (indicated by an arrow). Note the presence of presynaptic signal in the mushroom bodies, which is due to the background noise from the trans-Tango components and not the Lk-GAL4. (C, E) Lkr homozygous mutants show increased DILP2 immunoreactivity in insulin-producing cells (IPCs) of the adult brain. (D, F) Both Lk and Lkr homozygous mutants show increased DILP3 immunoreactivity in IPCs of the adult brain. (*** p < 0.001, **** p < 0.0001, as assessed by one-way ANOVA followed by Tukey’s multiple comparisons test). CTCF, corrected total cell fluorescence.