Table 2. Primers used for qPCR.
Primer | Sequence (5’ to 3’) |
---|---|
Primers for Lk and Lkr transcripts | |
Lk forward | GCCTTTGGCCGTCAAGTCTA |
Lk reverse | TGAACCTGCGGTACTTGGAG |
Lkr forward | GGAGGAAGCAGAATTTGAGCG |
Lkr reverse | AAAGTGTTGCCAATGACGGC |
Actin5C forward | AGCGCGGTTACTCTTTCACCAC |
Actin5C reverse | GTGGCCATCTCCTGCTCAAAGT |
β-tubulin forward | GCAGTTCACCGCTATGTTCA |
β-tubulin reverse | CGGACACCAGATCGTTCAT |
Primers for DILP2, 3 and 5 transcripts | |
DILP2 forward | CTCAACGAGGTGCTGAGTATG |
DILP2 reverse | GAGTTATCCTCCTCCTCGAACT |
DILP3 forward | CAACGCAATGACCAAGAGAAC |
DILP3 reverse | GCATCTGAACCGAACTATCACTC |
DILP5 forward | ATGGACATGCTGAGGGTTG |
DILP5 reverse | GTGGTGAGATTCGGAGCTATC |
RpL32/Rp49 forward | AGTATCTGATGCCCAACATCG |
RpL32/Rp49 reverse | CAATCTCCTTGCGCTTCTTG |