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. 2018 Nov 20;14(11):e1007767. doi: 10.1371/journal.pgen.1007767

Table 2. Primers used for qPCR.

Primer Sequence (5’ to 3’)
Primers for Lk and Lkr transcripts
Lk forward GCCTTTGGCCGTCAAGTCTA
Lk reverse TGAACCTGCGGTACTTGGAG
Lkr forward GGAGGAAGCAGAATTTGAGCG
Lkr reverse AAAGTGTTGCCAATGACGGC
Actin5C forward AGCGCGGTTACTCTTTCACCAC
Actin5C reverse GTGGCCATCTCCTGCTCAAAGT
β-tubulin forward GCAGTTCACCGCTATGTTCA
β-tubulin reverse CGGACACCAGATCGTTCAT
Primers for DILP2, 3 and 5 transcripts
DILP2 forward CTCAACGAGGTGCTGAGTATG
DILP2 reverse GAGTTATCCTCCTCCTCGAACT
DILP3 forward CAACGCAATGACCAAGAGAAC
DILP3 reverse GCATCTGAACCGAACTATCACTC
DILP5 forward ATGGACATGCTGAGGGTTG
DILP5 reverse GTGGTGAGATTCGGAGCTATC
RpL32/Rp49 forward AGTATCTGATGCCCAACATCG
RpL32/Rp49 reverse CAATCTCCTTGCGCTTCTTG