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. 2018 Nov 20;20:138. doi: 10.1186/s13058-018-1063-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — MMTV-Wnt1/dnIGF-1R tumors have altered immune cell infiltration. a, b Representative dot plot derived from flow cytometry measuring leukocyte marker CD45-PE/Cy5 of purified immune cells from Wnt1 (a) and Wnt1/dnIGF-1R (b) tumors. c Quantification of flow cytometry of CD45⁺ leukocytes in Wnt1 versus Wnt1/dnIGF-1R tumors (Wnt1/dnIGF-1R versus Wnt1 **P < 0.01; n = 20 each group). d, e representative dot plot of flow cytometry using cell surface markers to select for helper/regulatory T cells (CD4⁺CD45⁺) or cytotoxic T cells (CD8⁺CD45⁺) in Wnt1 (d) or Wnt1/dnIGF-1R (e) tumors. f, g Quantification of flow cytometry of CD4⁺CD45⁺-stained T cells (f) or CD8⁺CD45⁺-stained cytotoxic T cells (g) in Wnt1 versus Wnt1/dnIGF-1R tumors (CD4⁺CD45⁺ Wnt1/dnIGF-1R cells versus Wnt1 cells P > 0.05 (ns, not significant); CD8⁺CD45⁺ Wnt1/dnIGF-1R cells versus Wnt1 cells ***P < 0.001; n = 20 each group). h, i representative dot plot of flow cytometry using monocyte marker CD11b-PerCP of purified immune cells from Wnt1 (h) and Wnt1/dnIGF-1R (i) tumors. j Quantification of flow cytometry of CD11b⁺ monocytes in Wnt1 versus Wnt1/dnIGF-1R tumors (Wnt1/dnIGF-1R versus Wnt1 *P < 0.05; n = 20 each group)