Fig. 5.

Knockdown of C-C motif chemokine ligand 2 (CCL2) decreases monocyte migration towards A12-treated MCF7 cells. a, b Representative chart of RAW264.7 monocyte migration depicted as delta cell index over time (in hours) towards untreated (NT; black), human IgG-treated (IgG; red), or IMC-A12-treated (A12; blue) MCF7 cells (a) and quantification at 24 h (b) (one-way analysis of variance (ANOVA): *P < 0.05; n = 3, with four technical replicates in each experiment). c, d RT-PCR analysis of CCL2 (c) and IL-6 (d) in IgG-treated (CT) or A12-treated MCF7 cells after transfection with non-targeting scramble (Scr) or target-specific small interfering RNA (siRNA) (siIL-6, siCCL2) (one-way ANOVA: *P < 0.05, **P < 0.01, ****P < 0.0001; n = 3, with three biological replicates per experiment). e chart of RAW264.7 monocyte migration depicted as delta cell index over time (in hours) towards human IgG-treated (IgG; black), siScramble + A12-treated (Scr + A12; blue), siIL-6 + A12-treated (IL-6 KD + A12, red), or siCCL2 + A12-treated (CCL2 KD + A12, green) MCF7 cells (one-way ANOVA; n = 3, with four technical replicates in each experiment). f RT-PCR analysis of matrix metalloproteinase (MMP)-2, MMP-3, and MMP-9 in Wnt1/dnIGF-1R versus Wnt1 CD11b⁺ monocytes (Student’s t test *P < 0.05, **P < 0.01, ****P < 0.0001; n = 4, with three technical replicates per sample). GAPDH, glyceraldehyde-3-phosphate dehydrogenase