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. Author manuscript; available in PMC: 2018 Nov 20.
Published in final edited form as: Mol Microbiol. 2018 Apr 11;108(5):519–535. doi: 10.1111/mmi.13947

Figure 6. TgMAF1RHb1 mutants bind to SAM50.

Figure 6.

(A) Immunoprecipitation of lysed HFFs infected with cloned TgMe49: TgMAF1RHb1 16 C-terminal mutants, TgMe49:TgMAF1RHb1 and TgMe49:EV. Lysates were incubated with HA-conjugated beads and eluted with LDS sample buffer. Western blotting analysis was performed with the listed primary antibodies and HRP-conjugated secondary antibodies. Each 16 C-terminal mutant is able to bind to SAM50 (B) Densitometric input/eluate quantification of SAM50 western blot in panel A using ImageJ (NIH). Pulldowns of TgMAF1RHb1 C-terminal mutant infections have been repeated three times. Pulldowns of TgMAF1RHa1 infection was only performed once.