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. 2018 Nov 20;14:359. doi: 10.1186/s12917-018-1644-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The specificity of the FMDV RPA-LFD assays. Other bovine viral pathogens with similar clinic and etiologies were used to assess the specificity of the assays. There was no cross-reaction with BVDV, IBRV, BEV, BEFV, BVSV and SVDV. NC: negative control. A: primers/probe set of FMDV serotype A. AS: primers/probe set of FMDV serotype Asia 1. O: primers/probe set of FMDV serotype O. Samples were tested in triplicate with one reaction and three separate assays