Figure 3. Subcellular fractionation of wild type (N2) worms and worms expressing 40 polyglutamine repeats fused to YFP (Q40-YFP, AM141).

A representative immunoblot was probed with α-GFP antibody to detect polyglutamine. LMN-1 (nuclear marker) and α-tubulin (cytosolic marker) were used as controls. More details of the experiment can be found in Sin et al. (2017) .