Running Gel	Stacking Gel
dH2O	2.2 ml	1.74 ml
Tris-HCl (1.5 M, pH 8.8)	1.25 ml
Tris-HCl (0.5 M, pH 6.8)		0.625 ml
40% acrylamide/bis (29:1)	1.5 ml	0.25 ml
10% SDS	50 µl	25 µl
10% APS	25 µl	25 µl
TEMED	2.5 µl	2.5 µl