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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Carbohydr Polym. 2018 Oct 28;205:385–391. doi: 10.1016/j.carbpol.2018.10.071

Figure 4.

Figure 4.

Suramin inhibition of HPSE using the one-step FRET quenching assay. The experiments were performed in microplate format (100 μL) in 20 mM sodium acetate buffer, pH 5.0, containing 1 mg/mL heparin–DE and 1 μM HPSE at 37 °C for 4 h in the presence of varying concentrations of suramin. Solid lines represent curve fitting to standard dose-response equation.