Figure 6. Hypoxia suppresses BCAA catabolism and mmBCFA synthesis in adipocytes.

a. Volcano plot of changes in metabolite abundance in 3T3L1 cells exposed to hypoxia (1% O₂) or normoxia for 48 hours. 12 cell replicates. b. Glucose and amino acid uptake by 3T3L1 cells in hypoxia (1%) or normoxia. 14 cell replicates for lactate and glucose, 6 cell replicates for glutamine and glutamate, 3 cell replicates for leucine, isoleucine and valine. Two-tailed students t-test was performed for each comparison in a and b with no adjustment for multiple comparisons. c. Mole percent enrichment (MPE) of TCA intermediates from [U-¹³C] BCAAs in 3T3L1s following 24 hours of tracing in hypoxia (1%) or normoxia. d. Relative contribution of different substrates to lipogenic acetyl-CoA in 3T3L1s as determined by ISA following 24 hours of tracing in hypoxia (1%) or normoxia. e. % of each FA newly synthesized over 24 hours in hypoxia (1%) or normoxia. Three cell replicates are shown in c-e. All experiments in figure have been repeated 3 independent times with the same results. Data are presented as means ± SEM with dot plots overlaid with exception of b where data is presented as box (25^th to 75^th percentile with median line) and whiskers (min to max values) and d-e, mean +/− 95% confidence interval.