Fig 11. Identification of the underlying Boolean function of the regulatory effects of GAL4 and GAL80 on SWI5 in a synthetic network in Yeast.

A: The full schematic diagram of the synthetic network. B: The regulatory interactions in the absence of galactose (culturing cells in glucose) and the corresponding SPSD and the identified Boolean function, i.e., (GAL4)AND($\overline{GAL\mathit{80}}$). In the SPSD, RF₁ and RF₂ refer to GAL4 and GAL80, respectively. C-D: The regulatory interactions in the presence of galactose in the cases of the dissociation and the non-dissociation model, respectively. In both cases of C and D, the identified Boolean function only reflects the activatory effect of GAL4 on SWI5, i.e., GAL4→SWI5, despite the existing PPI between Gal4 and Gal80 in D.