Video 1. Timelapse movie of a single clonal DN2 progenitor lineage.

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DOI: 10.7554/eLife.37851.007

Bcl11b-YFP^-mCh^- DN2 progenitors were cultured on OP9-DL1 monolayers with 5 ng/mL IL-7 and Flt3-L within individual PDMS micro-wells, and continuously imaged for 100 hr. Images show superposition of a DIC image (gray) and cellular fluorescent intensities from the Bcl11b-mCherry (red) and Bcl11b-YFP (green) channels, with segmented cell boundaries shown in white. For clarity, images show only the fluorescence intensities within the cell boundaries, excluding auto-fluorescence from well boundaries and OP9-DL1 monolayers. Scatter-plot (bottom-right) updates with each frame to show fluorescent intensities of segmented cells at corresponding time points. Scale bar = 50 microns.