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Proteoglycans present in cell lysates of wild-type (WT) and SLC35B4-deficient (B4KO) cells were subjected to SDS-PAGE, transferred onto nitrocellulose membrane and probed with anti-chondroitin-4-sulfate (C4S) (A) or keratan sulfate (KS) (B) antibodies. Protein loading was assessed using Coomassie Brilliant Blue G-250 (CBB) staining (C).
