Table 2. List of expression plasmids constructed and used in this study.
Plasmid name | Original vector | Insert |
---|---|---|
pSelect-HA-SLC35B4 | pSelect-zeo (InvivoGen) | Human SLC35B4 with HA tag at the N-terminus |
pSelect-SLC35B4-HA | pSelect-zeo (InvivoGen) | Human SLC35B4 with HA tag at the C-terminus |
pSelect-HA-SLC35B4-K329A | pSelect-HA-SLC35B4 | Human SLC35B4 K329A with HA tag at the N-terminus |
pSelect-HA-SLC35B4-K330A | pSelect-HA-SLC35B4 | Human SLC35B4 K330A with HA tag at the N-terminus |
pSelect-HA-SLC35B4-K329AK330A | pSelect-HA-SLC35B4 | Human SLC35B4 K329AK330A with HA tag at the N-terminus |