Table 2. Antibodies for ICC used in this study.
Antibody Name | Reference Number | Opal Fluor | Reference Number |
---|---|---|---|
Rat anti-murine CD69 | R&D Systems (Clone#310116) Cat # MAB23861 |
Opal-520 | PerkinElmer FP1487001KT |
Rabbit anti-murine Notch1 | Cell Signaling Technologies (Clone-D1E11) Cat # 3608 |
Opal-690 | PerkinElmer FP1497001KT |
mono-Rat IgG1, K | BD Pharmingen (clone R3-34) Cat # 553922 | Opal-520 | PerkinElmer FP1487001KT |
mono-Rabbit IgG1 | Cell Signaling Technologies (Clone-DA1E) Cat # 3900S |
Opal-690 | PerkinElmer FP1497001KT |
Rat anti-human CD209 | LifeSpan BioSciences, Inc (Clone: h209) Cat # LS-B3782/73607 |
Opa-520 | PerkinElmer FP1487001KT |
mono-Rat IgG2a, k | Pharmingen (Clone R35-95) Cat # 553927 |
Opal-520 | PerkinElmer FP1487001KT |