Fig. 5.

H19 could bind to HIF-1α gene promoter region and increase the expression of HIF-1α by triggering HIF-1α to recruit EZH2 and H3K4me3. a the location of H19 in fibroblasts detected by FISH; b the binding of H19 to HIF-1α gene promoter region predicted by bioinformatics website; c dual luciferase reporter gene assay further verified the targeting relationship of H19 to HIF-1α; d-e enrichment of H3K4me3 in HIF-1α gene promoter region by ChIP assay; f-g expression of HIF-1α after silencing and overexpressing H19; h enrichment of H3K4me3 in HIF-1α gene promoter region after EZH2 knockout by ChIP assay; i-j expression of HIF-1α in fibroblasts after EZH2 knockout by western blot analysis; *, p < 0.05, compared with the NC group or normal group; #, p < 0.05, compared with the sh-H19 NC group; &, p < 0.05, compared with the oe-H19 NC group. The above results were measurement data, which were presented as the mean ± standard deviation. Comparisons between two groups were analyzed by t-test. The experiment was repeated 3 times; ChIP, Chromatin Immunoprecipitation; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; EZH2, enhancer of zeste homolog 2; NC, negative control; HIF-1α, hypoxia-inducible factor-1α; FISH, fluorescence in situ hybridization