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. 2018 Nov 20;10:115. doi: 10.1186/s13195-018-0441-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Amyloid-β (Aβ) immunization results in removal of amyloid plaques in brains of triple-transgenic Alzheimer’s disease (3xTg-AD) mice. Brain sections of mice aged 18 months (c and d) and 20 months (a, b, e–h) were stained with a NeuN antibody (red) to detect neurons and an anti-Aβ antibody (McSA1, brown) to detect numerous plaques in the subiculum of the hippocampus in 3xTg-AD mice. a The hippocampus of a 20-month-old female control 3xTg-AD mouse with numerous amyloid plaques is shown (5× magnification). b Hippocampus of a 20-month-old male control 3xTg-AD mouse showing no plaque pathology. c The subiculum of an 18-month-old female control 3xTg-AD mouse is shown at higher magnification (20×). d Aβ staining in the subiculum of an 18-month-old male mouse. Only intraneuronal Aβ can be detected (indicated with arrow and shown at higher magnification in inset). e Numerous plaques in the hippocampus of an untreated 20-month-old 3xTg-AD female mouse. f No plaques were found in 20-month-old wild-type mice. Both immunization regimens, Aβ₄₂ peptide (g) and DNA Aβ₄₂ (h), led to a reduction of plaques in 20-month-old 3xTg-AD mice compared with the control mouse (e). i Images were counted for plaques ≥ 10 μm in a 1-mm² area of the subiculum/CA1 of the hippocampus by two blinded experimenters. Blue bars show plaque count in DNA Aβ₄₂ trimer-immunized mice (n = 7), and yellow bars show plaque count found in brains of Aβ₄₂ peptide-immunized mice (n = 8). Black bars show the numbers found in age- and gender-matched 3xTg-AD control mice (n = 15). * indicates p value of ≤ 0.05 (unpaired Student's t test)