Fig 2. Design of CVV and KRIV that do not express NSs.

Shown are sections of S segment for the N-termini of the N and overlapping NSs proteins. Mutations were added to disrupt the reading frames of the NSs proteins for both viruses, without changing the amino acid sequence of the overlapping N protein. For CVV, two methionines were changed to threonine (denoted in bold) and two stop codons introduced (denoted with an asterisk). For KRIV, mutational sites were used where three methionines were changed to threonines and two stop codons introduced. Protein representation is not to scale.