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. 2018 Oct 29;12(10):e0006884. doi: 10.1371/journal.pntd.0006884

Fig 5. Type I IFN protection assay for orthobunyaviruses.

Fig 5

A549 cells were infected with rBUNV, rCVV, rKRIV or rCVVdelNSs, rBUNVdelNSs (clone rBUNVdelNSs2) or rKRIVdelNSs at a MOI of 1 and incubated at 37 °C for 48 hrs. Twofold dilutions of the clarified and UV-treated supernatant were used to treat fresh A549 NPro cells in a 96-well plate for 24 hrs. The cells were then infected with EMCV and the development of CPE was monitored at 96 hrs post-infection by staining with crystal violet. The production of IFN was calculated according to the highest dilution of supernatant giving protection against EMCV infection and is expressed as relative IFN units. The experiment was conducted three times and gave identical results.