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. 2018 Oct 29;12(10):e0006884. doi: 10.1371/journal.pntd.0006884

Fig 8. Attempted rescues of CVV and KRIV M segment reassortants.

Fig 8

Western blot analysis showing the lack of N protein produced in 3 independent rescues for the hybrid viruses CKC and KCK (with segment order S-M-L). Supernatant from rescue experiment was passaged (1 ml of rescue supernatant added to BHK-21 cells for 5–7 days) and cell lysates used for western blotting using an anti-CVV N antibody for the hybrid CKC and an anti-KRIV N antibody for KCK. +ve 1, rCVV-infected cell lysate; +ve 2, rKRIV-infected cell lysate; U, uninfected cells.