Fig. 4.

In vitro suppressive function assay from bone marrow of newly diagnosed MM patient. Representative curves are shown for a MM patient. a Negative control: unlabeled CD4⁺ CD25⁻ T cells alone. b Positive control 1: CFSE-labeled CD4⁺ CD25⁻ T cells without anti-CD3/CD28 beads. c Positive control 2: CFSE-labeled CD4⁺ CD25⁻ T cells with anti-CD3/CD28 beads. d aTreg group: CFSE-labeled CD4⁺ CD25⁻ T cells cocultured with aTreg at a ratio of 1:1. e rTreg group: CFSE-labeled CD4⁺ CD25⁻ T cells cocultured with rTreg at a ratio of 1:1. f non-Treg: CFSE-labeled CD4⁺ CD25⁻ T cells cocultured with non-Treg at a ratio of 1:1