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. 2018 Nov 20;19:828. doi: 10.1186/s12864-018-5201-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Identification of exosomes in PM. a The morphology of PM. b 2D AFM image of isolated exosome vesicles in PM. c 3D AFM image of isolated exosome vesicles in PM. d The line profile of AFM image for a PM exosome vesicle. X- and Y-axes are the width and height of PM exosome vesicle, respectively. e The expression of CD63 and Tubulin in the crop tissue and exosome vesicles was determined by Western blotting. It can be seen that the isolated exosome vesicles are positive for CD63 but negative for Tubulin