Table 3.

Identified putative AMP sequences with no significant identity to the known proteins, less than 80% identity to known AMP sequences or 100% identity to proteins stored at NCBI database with < 70% query cover and their physico-chemical characteristics

#	Sequence	Species	Charge	Ha
No identity to proteins stored at NCBI nr database
1	FLGALGNALSRVLGK	R. temporaria	+3	0.813
2	FIGALVNALTRVLGK	R. temporaria	+3	1.213
3	FIGALVHALTGILGK	R. temporaria	+ 2	2.247
4	LVPFIGRTLGGLLARFGK	R. temporaria	+ 4	1.500
5	VPQLCFKFQKVIYCEINRTLPNEA	R. dalmatina	+ 2	−0.625
6	FSQLFFAWLLRLCRQ	P. kl. esculentus	+3	2.587
7	GIVEAWPLR	P. ridibundus, R. dalmatina	+ 1	1.133
8	NNLRHIVAWCKNRNYSLAVCARFKPQ	R. temporaria	+ 6	−1.612
9	NLLGFLQGAKDILKECEADNYQGWLCESYKPQ	R. dalmatina	−1	−1.250
10	FLPLVLGKTHSEQAEILSWKSSNVEYHLPKCTTDV	P. ridibundus, R. dalmatina	0	−0.763
11	FLPLIAGLWVNCSANNPKMLKLWK	P. kl. esculentus	+ 4	1.363
12	FLPICDKSALRFVGKV	P. ridibundus	+3	0.494
13	EMPMKKKEETIQKKGMLKWKTIFTSHCWSFE	P. ridibundus	+ 4	−1.835
14	RGLLDPITGLVGGLLR	H. arborea	+ 2	1.213
< 80% identity to proteins stored at NCBI database
15	FLGFVGQALNALLGKLGK	R. dalmatina	+3	1.550
16	FLPAIAGILSQIFGK	P. kl. esculentus	+ 2	2.540
17	FFPAFLKVAAKVVPSIICSITKNVET	P. kl. esculentus	+3	0.573
18	IVPILLGVVPQLVCAITKKC	R. dalmatina, R. temporaria	+3	1.675
19	IIPLLLGKVVCAITKKC	R. dalmatina	+ 4	1.271
20	LVPMFLSKLICFITKKC	R. temporaria	+ 4	1.918
21	GLEVLGKILSGILGK	R. dalmatina, Rana arvalis	+ 2	1.220
22	LLGAALSALSSVIPSVISWFQKG	Rana arvalis	+ 2	1.670
23	LANRAARNTSQNVLNAITCTL	R. dalmatina	+3	−1.662
24	ADFLDKLRNFAAKNLQNKASL	P. ridibundus	+3	−1.595
25	EMLRKKEETIQKKGMLKWKNDFYQSLLEF	P. ridibundus	+3	−1.779
100% identity to proteins stored at NCBI database with < 70% query cover
26	QKTYNRRPPGWSLYVFHQQISNLELEVI	P. kl. esculentus	+ 2	−0.654
27	FVPLLVSKLVCVVTKNVRIWKLELEII	Rana arvalis	+3	1.663
28	FVPLLVSKLVCVVTKNVRTLET	Rana arvalis	+3	0.455
29	FLPIVTNLLLRFVG	R. dalmatina	+ 2	3.729

^aCalculated using the CCS consensus hydrophobicity scale [50]