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. 2018 Nov 19;49:114. doi: 10.1186/s13567-018-0610-2

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Western blot analysis of comparative proteomics data. A The left lane was loaded with bacterial proteins from M. hyopneumoniae strain 168. The right lane was loaded with bacterial proteins from strain 168L. The differentially abundant protein fructose-1,6-bisphosphate aldolase (FBA; 44 kDa) was analysed using the corresponding antibodies. Protein bands were visualised using Electro-Chemi-Luminescence (ECL) substrate. A Ponceau-S stained membrane was used as the loading control. B Image J software was used to calculate the optical density of the corresponding bands in the blots. The optical density of the corresponding bands was normalized to the total proteins of Ponceau-S staining of the same membrane. The level of abundance of FBA in M. hyopneumoniae strain 168L is expressed as the percentage of that in M. hyopneumoniae strain 168. The asterisk above the charts stands for statistically significant differences.