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. 2018 Nov 19;49:114. doi: 10.1186/s13567-018-0610-2

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of FBA on the surface of M. hyopneumoniae by flow cytometry. Negative control, M. hyopneumoniae strain 168 and 168L treated with preimmune serum; M. hyopneumoniae strain 168 and 168L: bacteria treated with anti-rFBA serum. The level of mean fluorescence intensity (MFI) of M. hyopneumoniae incubated with anti-rFBA sera is expressed as the percentage of the corresponding strain incubated with preimmune sera. The asterisks above the charts stand for statistically significant differences.