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. 2018 Nov 19;49:114. doi: 10.1186/s13567-018-0610-2

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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rFBA and fibronectin interaction analysis by Far-WB and Surface plasmon resonance (SPR) analysis. A Far-WB analysis of rFBA with fibronectin. The first lane: PVDF membrane with transferred rFBA protein incubated with anti- rFBA antibody as a positive control; the second lane: PVDF membrane with transferred rFBA protein incubated with fibronectin and anti-fibronectin antibody; the third lane: PVDF membrane with transferred BSA (negative control) incubated with fibronectin and anti-fibronectin antibody. Protein bands were visualized using ECL substrate. B Sensorgrams depict the binding of immobilised fibronectin to rFBA. Increasing concentrations of rFBA (5, 10, 25, 50 and 100 μg/mL) were injected at a flow rate of 30 μL/min for 180 s over immobilised fibronectin. The arrow indicates the end of the injection period, at which point dissociation of rFBA from fibronectin can be observed. RU resonance units.