Table 1.
The proteins with significant changes in abundance
| Spot no. | Protein description | Fold change | Mascot scorea | Theoretical pI/Mw | Sequence coverage (%) | Accession |
|---|---|---|---|---|---|---|
| 1 | Molecular chaperone DnaK (DnaK) | + | 179 | 5.17/68281.56 | 6 | ADQ90292.1 |
| 2 | Elongation factor Tu (EF-Tu) | 1.88 | 711 | 5.61/44122.49 | 27 | ADQ90729.1 |
| 3 | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) | + | 198 | 6.67/36873.02 | 11 | ADQ90256.1 |
| 4 | Adenine phosphoribosyltransferase (Apt) | 1.94 | 216 | 5.34/18849.21 | 22 | ADQ90402.1 |
| 5 | l-Lactate dehydrogenase (LDH) | 1.72 | 442 | 8.29/34237.25 | 20 | ADQ90382.1 |
| 6 | Heat shock protein (GrpE) | 1.97 | 87 | 5.47/28854.10 | 6 | ADQ90239.1 |
| 7 | Fructose-bisphosphate aldolase (FBA) | + | 342 | 9.05/43780.88 | 20 | ADQ90241.1 |
aMascot computes a score based on the probability that the peptides from a sample match those in the selected protein database. The more peptides Mascot identifies from a particular protein, the higher the Mascot score for that protein.
“+” Means those proteins that only observed in the 2-DE map of M. hyopneumoniae strain 168, which means its expression level in strain 168L is very low.