Fig. 2.

Increased autoantibody production in naive WASp^−/− and WASp^−/−N-WASp^fl/flCD19^Cre+ mice. Serum from naïve mice and mice immunized with apoptotic cells were screened for reactivity to 95 different autoantigens using autoantibody array. Autoantigens are sorted by ANOVA starting with lowest p-value at the top for (A) IgM autoantibodies and for (B) IgG autoantibodies. Serum from 7 to 9 mice were tested, data of individual mice are shown. Black color equals ⩽ 1-fold change as compared with average values in control serum (WT d 0). A yellow square indicates a ⩾10-fold increase of autoantibody titer and a black square no difference as compared with average values in control serum. All yellow nuances in between represent a value larger than 1 and smaller than 10. WT, WASp^−/−, WASp^−/−N-WASp^fl/flCD19^Cre/+ d 0 n = 7, WT, WASp^−/−N-WASp^fl/flCD19^Cre/+ d 27 n = 9. (C–D) Autoantibodies in 8–10 weeks old mice. Serum titers of anti-DNA and anti-chromatin (C) IgM and (D) IgG measured in 8–10 weeks old unchallenged mice. WT n = 3, WASp^−/− n = 7, WASp^−/−N-WASp^fl/flCD19^Cre/+ n = 7, each dot correspond to one mouse. Significance was assessed with unpaired, two-tailed Student t test. *P < 0.05. Outliers based on ROUT (Q = 1%) excluded: (C) 2 cDKO anti-DNA, 1 cDKO anti-chromatin (D) 1 WKO anti-DNA, 1 WKO anti-chromatin. Abbreviations: WKO; WASp^−/−, cDKO; WASp^−/−N-WASp^fl/flCD19^Cre/+.