Fig. 4.

Increased retention and decreased proliferation of WASp^−/− and WASp^−/−N-WASp^fl/flCD19^Cre/+ GC B cells. (A) Proliferating cells were labeled after the third and fourth immunization with apoptotic cells by providing the nucleotide analogous EdU and BrdU in the drinking water for 6 subsequent days. (B and C) Flow cytometry data of (B) EdU⁺ or (C) BrdU⁺ B cells in GCs at d 27. n = 5–6. Note that the gates appear slightly altered because of the staining procedure for EdU and BrdU. Significance was assessed with unpaired, two-tailed Student t test. *P < 0.05, **P < 0.01. Abbreviations: WKO; WASp^−/−, cDKO; WASp^−/−N-WASp^fl/flCD19^Cre/+.