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. Author manuscript; available in PMC: 2018 Nov 20.
Published in final edited form as: J Autoimmun. 2015 Jul 2;62:81–92. doi: 10.1016/j.jaut.2015.06.003

Fig. 7.

Fig. 7.

Altered B cell affinity maturation in WASp−/−N-WASpfl/flCD19Cre/+ mice. (A and B) Characterization of replacement mutations found in the VH1-186.2 family of (A) IgM and (B) IgG1. Grey background highlights the CDR1, CDR2, and CDR3 regions. Pink arrowhead indicates the high affinity amino acid mutation W33L. Amount of unique clones analyzed; WT Cμ n = 34, WT Cγ1 n = 109, WKO Cμ n = 36, WKO Cγ1 n = 34, cDKO Cμ n = 80, cDKO Cγ1 n = 21. (C) Breakdown of peripheral tolerance inWASp deficiency. N-WASp activity in WASp-deficient B cells supports increased reactivity to self antigens. Abbreviations: WKO; WASp−/−, cDKO; WASp−/−N-WASpfl/flCD19Cre/+.