Fig. 2. CV-B5 infection in 3-day-old mice resulted in dose-dependent disease and mortality.

Three-day-old BALB/c mice (n = 6 to 10, per group) were i.p. inoculated with CV-B5/JS417 at a dose ranging from 3.16 to 3.16 × 10⁵ CCID₅₀/mouse (10-fold serially diluted). The control mice were treated with medium. The CV-B5/JS417-induced mean score of clinical symptoms and CV-B5/JS417-induced mortality were monitored and recorded daily. Representative results of duplicate experiments are shown. The Mantel–Cox log-rank test was used to compare the survival of the pups between each group and the medium control group at 21 days post-infection. ***p < 0.001. *p < 0.05